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Journal: iScience
Article Title: Prognostic value of BTG1 for predicting decitabine sensitivity in de novo acute myeloid leukemia
doi: 10.1016/j.isci.2025.114327
Figure Lengend Snippet: Downstream signaling pathway of BTG1 in AML cell lines (A) Top three signaling pathways in terms of Gene Ratio were EBV infection (0.037), Wnt signaling pathway (0.035), and hematopoietic lineage (0.023) of KEGG pathway enrichment. (B) Effect of BTG1 on the mRNA expression of β-catenin, Cyclin D1, and C-Myc. (C) Effect of BTG1 on the protein expression of β-catenin and Cyclin D1. (D) The relative viability of AML cells after interference of BTG1 and treatment with FH535. (E) Apoptosis of AML cells after interference of BTG1 and treatment with FH535. Data are presented as mean ± standard deviation (SD). (ns, p > 0.05; ∗ p < 0.05; ∗∗ p < 0.01; ∗∗∗ p < 0.001; ∗∗∗∗ p < 0.0001).
Article Snippet:
Techniques: Protein-Protein interactions, Infection, Expressing, Standard Deviation
Journal: Animal Bioscience
Article Title: Transcriptomic analysis identifies CXCL12 as a novel candidate gene for litter size in rabbits
doi: 10.5713/ab.24.0640
Figure Lengend Snippet: CXCL12 overexpression and knockdown in GCs. (A,B) Changes in the expression of CXCL12 after its overexpression and knockdown in GCs. (C,D) The mRNA expression level of PCNA , CCND1 , CDK2 , Bcl-2 and Bax was detected after overexpressing or knocking down CXCL12 in GCs. (E) The protein expression levels of CCND1, PCNA, Bcl-2 and Bax were detected after overexpressing and knocking down CXCL12 in GCs. H, high-litter size group; L, low-itter size group; GAPDH was selected as the internal reference gene (The t test was used for the above analyses comparing two individual samples. * p<0.05; ** p<0.01; *** p<0.001; ns, not significant). GAPDH, glyceraldehyde-3-phosphate dehydrogenase; GC, granulosa cells.
Article Snippet: Protein detection was achieved using the following antibodies:
Techniques: Over Expression, Knockdown, Expressing